Antibodies are immune system proteins that bind to antigens and can be used for many applications in research, medicine, diagnostic testing, and therapy. Antibodies can be produced using several different methods, and one of the most effective methods is hybridoma technology. Hybridoma production involves fusing B cells and myeloma cells, which results in the generation of monoclonal antibodies (mAbs) against a specific antigen.

SYnabs has developed a variety of strategic immunization types and injection schedules for optimal antibody production. By directing the immunized animal’s antibody response with smart antigen design, SYnabs ensures that you obtain high-affinity, high-specificity, and high-titer antibodies for your application needs. Read more bosterbio.com

We can produce custom monoclonal antibodies based on your target antigen or an existing human antibody. We can also provide custom peptides that can be used as immunogens for your immunization study.

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Once a high-affinity, high-specificity, or high-titer antibody is generated, we can analyze the antiserum for unique functional properties such as neutralizing activity, inhibitory activity, or agonist activity. If your antibody shows desirable properties, we can further purify the antibody and perform additional analysis on it such as western blot, cytofluorescence, or other immunological assays.

Once the initial screening of hybridoma clones has been conducted, investigators will choose up to two clones to be subcloned and assayed for monoclonality and stability. Each positive clone will be characterized in your lab for additional functional assays and if required, further protein expression and purification services can be performed to create a reagent suitable for your downstream applications.